Drugs Exp Clin Res. 1996;22(3-5):213-7.
Theoretical grounds and experimental confirmation of the antiviral effect of the
Lozjuk RM, Lisnyak OI, Lozjuk LV.
Department of Pharmacology, Medical Institute, Ukraine.
The preparation Ukrain was studied in order to confirm its theoretically
substantiated properties in comparison with amitozin as its structural and
functional analogue, and two other functional analogues, namely methisazone
(against herpesvirus and poxvirus) and remantadine (against grippevirus). The
experiments were carried out on albino Swiss mice of either sex weighing
initially 18-20 g. They were kept at room temperature (20-21 degrees C) on a
natural day-night cycle, were housed in groups of ten per cage and had free
access to food and water. The Ukrain preparation's activity as concerns grippe
virus of A type (England) 42/72 (H3N2) was determined in ovo and in vivo, the
treatment beginning 2 h after infection. The control animals, infected by
appropriate virus dose, were injected with placebo. For the activities of the
remedies investigated, our estimate was based on the median duration of life in
the mice of the subexperimental and control groups (duration of experiments 14
days), the degree of intensity of pulmonary tissue changes, and the difference in
median HR titres. The totality of Chelidonium majus alkaloids, thiophosphamide
(the alkalizing agent), the sum of alkaloids modified by thiotepa, and some other
alkaloids as their alkalized derivatives, all showed a statistically reliable
inhibiting effect. The most significant biological activity was demonstrated by
Ukrain as concerns the models of viral processes, and by the sum of alkaloids and
amitozin as concerns the models of associated infections. This coincides with the
results of biological assessment of modified products (1, 3, 5) with metabolites,
alkaloids in particular, or alkaline substances.