Cancer Lett. 2009 Jul 7.
acid, a major component of Toona sinensis leaf extracts, contains a
ROS-mediated anti-cancer activity in human prostate cancer cells
Chen HM, Wu YC, Chia YC, Chang FR, Hsu HK, Hsieh YC, Chen CC, Yuan SS.
Institute of Natural Products, College of Pharmacy, Kaohsiung Medical
University, Kaohsiung 807, Taiwan, ROC; Department of Medical Research,
E-DA Hospital, Kaohsiung 824, Taiwan, ROC.
cancer, the most frequently diagnosed malignancy in elderly males of
the United States, has become a major health issue in Asia. Previous
studies have demonstrated that leaf extracts of Toona sinensis Roem.
contain cytotoxic activity on several cancer cells including prostate
cancer cells. In this study, gallic acid is identified as the major
anti-cancer compound in T. sinensis leaf extracts. It is cytotoxic to
DU145 prostate cancer cells, through generation of reactive oxygen
species (ROS) and mitochondria-mediated apoptosis, which were reversed
by antioxidants catalase and N-acetylcysteine. Furthermore, gallic acid
is shown to block the growth of DU145 cells at G2/M phases by
activating Chk1 and Chk2 and inhibiting Cdc25C and Cdc2 activities. In
addition, gallic acid has a synergistic effect with doxorubicin in
suppressing the growth of DU145 cells. Taken together, our results
suggest that gallic acid has the potential to be developed into an
anti-prostate cancer drug and is worthy of further studies.